Projects & Grants
| DNA damage repair gene expression in pulmonary large-cell neuroendocrine carcinoma | |
|---|---|
| Project Id | |
| Main solver | Mgr. Adam PUT |
| Period | 1/2025 - 12/2025 |
| Provider | Specifický VŠ výzkum |
| State | solved |
| Anotation | Large-cell neuroendocrine carcinoma of the lung (LCNEC) poses a considerable challenge due to its aggressive nature and the current lack of effective treatment options. Recent research has focused on the role of DNA damage repair genes in determining the efficacy of radio- and chemotherapy. This project aims to characterize the expression profiles of key oncogenes, specifically BRCA1, BRCA2, and RAD51, which are critical for the repair of DNA double-strand breaks through homologous recombination. Based on existing in vitro studies using cell lines derived from LCNEC patients, the detection of these genes' expression may aid in identifying patients who are likely to benefit from chemotherapy and radiotherapy. Consequently, we will analyze BRCA1/2 and RAD51, as their increased expression has been associated with resistance to these treatments. Notably, the expression profile of these resistance biomarkers has not yet been investigated in LCNEC. The detection will be conducted using standard RT-PCR with TaqMan probes on a rare and unique retrospective set of 30 FFPE samples from LCNEC patients. This study will generate a comprehensive expression profile of DNA damage repair genes within this distinctive patient cohort. Should significant differences in the expression of key DNA repair genes be identified, the results will serve as a basis for an international multicenter project proposal. This proposed project will seek to validate the findings in a larger patient cohort and establish correlations with clinicopathological data. |
