Projects & Grants

Internal Grant Competition DGC
START-UP grant

Comparison results of DNA analysis for two types of biological materials after invasive and non-invasive sampling.
Project IdSGS05/LF/2020-2021
Main solverMgr. Petra Riedlová, Ph.D.
Period1/2020 - 12/2021
ProviderSpecifický VŠ výzkum
AnotationVarious of biological materials are used for DNA analysis, the sampling of which is either invasive (e.g. blood) or non-invasive (e.g. saliva). Currently are commonly used non-invasive sampling already , for example in forensic science or forensic medicine. However, in a various cases can ´t be used non-invasive sampling though the amount of DNA after isolation may be sufficient, but the analysis may be interfered with by a variety of other factors. The amount of DNA required for analysis depends not only on the type of biological material used, but also on the isolation and DNA analysis techniques. The amount of DNA obtained from both blood and saliva should be sufficient for the purpose of this study, i.e. 500 ng / ml of blood (saliva). For this study, we propose to compare the quantification of the gene coding Factor V Leiden located on the first chromosome (1q23). This gene occurs in the whole population and may occur in some individuals with a single nucleotide mutation c.1691G>A of the hemocoagulation factor gene. This variation may manifest like a impaired blood clotting process. In the Czech population, 5?10 % of the people carry of the FV Leiden mutation. Finding a given sequence variant increases the risk of clotting and vascular blocking many times, which in combination with hormonal contraceptives and smoking further multiplies. This may result in an increased risk of stroke, myocardial infarction, pregnancy problems, thrombosis or embolism at a young age. The result of the study will be the assessment of the rate of agreement of the results (quantification) of the examination of the given gene mutation performed in two types of biological materials. Participants of study will be informed of the results of the study and also of the results of their own DNA analysis.