Projects & Grants

Internal Grant Competition DGC

Estimation od reference intervals of selected synovial fluid biomarkers
Project IdSGS14/LF/2019-2020
Main solverMgr. Iveta Bystroňová
Periodr1/2019 - 12/2020
ProviderSpecifický VŠ výzkum
AnotationThe aim of the project is to estimate the reference interval for selected synovial fluid (ST) biomarkers. ST is made up of a blood plasma ultrafiltrate that is enriched with mucopolysaccharides produced by joint lining cells. Under physiological conditions, ST contains hyaluronate, electrolytes, glucose, proteins and enzymes. However, the exchange of substances between the vascular and lymphatic system and the ST may be impaired or altered. The ST analysis could therefore provide information about the type and degree of alteration in the joints. Examination of ST parameters in articular diseases could be a valuable diagnostic procedure. For ST, therefore, it is important to distinguish whether it is physiological, non-inflammatory, inflammatory, septic or haemorrhagic, as well as determining the infectious character of the joint disease (or the differentiation of the inflammatory and degenerative origin of joint problems), diagnosis of gout and chondrocalcinosis (pseudogout). We believe it is necessary to set reference intervals for basic biochemical (glucose, total protein, uric acid, lactate, CRP ...), haematological (number of white blood cells, number of red blood cells ...) and special parameters in synovial fluid (IL-6, presepsin, calprotectin ...). Biochemical parameters will be measured in a standard way on the AU 5800, haematological in Body Fluid on the Sysmex XN-9000 and special by immunochemical methods. It is also necessary to standardize the procedure and evaluation for the detection of crystals in the ST. Commonly, crystals are neither free nor intracellular. Acute gouty arthritis occurs in crystals of sodium urate, in acute chondrocalcinosis of calcium pyrophosphate and in rheumatoid arthritis of cholesterol. Crystal resolution will be performed by polarizing microscopy. This project would make a huge contribution to orthopedics and rheumatology, as patients with joint infectious diseases are constantly on the increase.