Projects & Grants

Internal Grant Competition DGC

Trypanosomatids with in-frame sense stop codons: dissecting molecular mechanisms behind the unique ambiguity of the genetic code
Project Id18-15962S
Main solverprof. MSc. Vjačeslav Jurčenko, Ph.D.
Periodr1/2018 - 12/2020
ProviderKatedra biologie a ekologie, Standardní projekt GA ČR
AnotationFor decades, the genetic code was considered to be universal, an immutable "inner sanctum" of the cellular machinery. Almost all life forms share the same code, and it is arguably still the most conserved molecular feature. Fortunately for scientists poking into the secrets of the genetic code, exceptions do exist and their dissection is very informative. We have recently described a unique variation in the poorly known monoxenous trypanosomatid Blastocrithidia sp. in which all three stop codons are re-assigned as coding ones. In this project we propose to dissect molecular mechanisms governing the unique ambiguity of the genetic code in this species. We will combine genomic, transcriptomic, and proteomic approaches with functional studies aimed to understand how the translation works in Blastocrithidia.